Semaglutide 20mg Reconstitution: How Much Bacteriostatic Water & How Many Units
A 20mg vial of Semaglutide contains 20,000 mcg. After you add bacteriostatic water, the amount of water decides the concentration — and therefore how many units you draw on a U-100 insulin syringe (1 ml = 100 units). The tables below cover the common water amounts.
Concentration by water amount
| Water added | Concentration | Per 1 unit (0.01 ml) | Per 10 units (0.1 ml) |
|---|---|---|---|
| 1 ml | 20 mg/ml | 200 mcg | 2 mg |
| 2 ml | 10 mg/ml | 100 mcg | 1 mg |
| 3 ml | 6.67 mg/ml | 66.67 mcg | 666.67 mcg |
How many units to draw
Example doses across the research range listed for Semaglutide (250 mcg–2.4 mg). Units are on a U-100 insulin syringe.
| Dose | With 1 ml | With 2 ml | With 3 ml | Doses per vial |
|---|---|---|---|---|
| 250 mcg | 1.3 units | 2.5 units | 3.8 units | 80 |
| 1 mg | 5 units | 10 units | 15 units | 20 |
| 1.5 mg | 7.5 units | 15 units | 22.5 units | 13 |
| 2.4 mg | 12 units | 24 units | 36 units | 8 |
How to mix it
- Wipe the vial stoppers with alcohol and let them dry.
- Draw your chosen amount of bacteriostatic water into a sterile syringe.
- Inject the water slowly down the inside wall of the Semaglutide vial — not directly onto the powder.
- Swirl gently until clear. Do not shake.
- Label the vial with the date and concentration, and store it in the refrigerator.
Full walkthrough: Peptide Reconstitution Guide · Injection Guide · Semaglutide research profile
FAQ
How much bacteriostatic water do I add to a 20mg Semaglutide vial?
Common choices are 1 ml, 2 ml, 3 ml. With 2 ml the concentration is 10 mg/ml. More water makes each dose a larger, easier-to-measure draw; less water keeps injection volume small. The amount of water does not change the dose — only how many units you draw.
How many units is 250 mcg of Semaglutide from a 20mg vial?
With 2 ml of bacteriostatic water, 250 mcg is 2.5 units on a U-100 (1 ml) insulin syringe. See the table above for other water amounts.
How many doses are in a 20mg Semaglutide vial?
At 250 mcg per dose, a 20mg vial holds about 80 doses. At higher doses it holds fewer — see the table.