Semaglutide 5mg Reconstitution: How Much Bacteriostatic Water & How Many Units
A 5mg vial of Semaglutide contains 5,000 mcg. After you add bacteriostatic water, the amount of water decides the concentration — and therefore how many units you draw on a U-100 insulin syringe (1 ml = 100 units). The tables below cover the common water amounts.
Concentration by water amount
| Water added | Concentration | Per 1 unit (0.01 ml) | Per 10 units (0.1 ml) |
|---|---|---|---|
| 1 ml | 5 mg/ml | 50 mcg | 500 mcg |
| 2 ml | 2.5 mg/ml | 25 mcg | 250 mcg |
| 3 ml | 1.67 mg/ml | 16.67 mcg | 166.67 mcg |
How many units to draw
Example doses across the research range listed for Semaglutide (250 mcg–2.4 mg). Units are on a U-100 insulin syringe.
| Dose | With 1 ml | With 2 ml | With 3 ml | Doses per vial |
|---|---|---|---|---|
| 250 mcg | 5 units | 10 units | 15 units | 20 |
| 1 mg | 20 units | 40 units | 60 units | 5 |
| 1.5 mg | 30 units | 60 units | 90 units | 3 |
| 2.4 mg | 48 units | 96 units | 144 units (over 1 ml — split or use a larger syringe) | 2 |
How to mix it
- Wipe the vial stoppers with alcohol and let them dry.
- Draw your chosen amount of bacteriostatic water into a sterile syringe.
- Inject the water slowly down the inside wall of the Semaglutide vial — not directly onto the powder.
- Swirl gently until clear. Do not shake.
- Label the vial with the date and concentration, and store it in the refrigerator.
Full walkthrough: Peptide Reconstitution Guide · Injection Guide · Semaglutide research profile
FAQ
How much bacteriostatic water do I add to a 5mg Semaglutide vial?
Common choices are 1 ml, 2 ml, 3 ml. With 2 ml the concentration is 2.5 mg/ml. More water makes each dose a larger, easier-to-measure draw; less water keeps injection volume small. The amount of water does not change the dose — only how many units you draw.
How many units is 250 mcg of Semaglutide from a 5mg vial?
With 2 ml of bacteriostatic water, 250 mcg is 10 units on a U-100 (1 ml) insulin syringe. See the table above for other water amounts.
How many doses are in a 5mg Semaglutide vial?
At 250 mcg per dose, a 5mg vial holds about 20 doses. At higher doses it holds fewer — see the table.